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Transgenic Organism - Transgenic Services Tailored To Your Needs
The creation of genetically modified mice requires a wide range of expertise. Transgenic Organism has had a high success level with both 129Sv/Ev and C57BL/6 ES cell lines.
Transgenic Organism creates Conventional and Conditional knockout mice, Knockin mice, and Conventional/Conditional knockout mice with a reporter gene. In addition, many investigators create their own targeting constructs and have Transgenic Organism perform the remaining steps, i.e. from electroporation onward.
 Conventional Knockout Mice: A mouse model in which a targeted gene has been functionally eliminated. These mice can be used to study the unique function of a specific gene within the animal's genome.
 Conditional Knockout Mice: A mouse model that utilizes the cre-lox system (site specific DNA recombination through the interaction of the Cre recombinase enzyme and lox-P sites in the DNA strand for the deletion of a gene in targeted tissues). Transgenic Organism has a license agreement to use the cre-lox technology with Bristol Myers Squibb.
 Knockin Mice: A mouse model in which the targeted gene sequence has been subtly altered to modify its function. Transgenic Organism can introduce specific point mutations or generate insertions/deletions according to the specific needs of the experiment.
 Conventional/Conditional Knockout Mice with Reporter Gene: A mouse model that contains the beta-galactosidase reporter gene (Lac-Z), which serves as a marker for target gene expression.
Outside Constructs: If investigators choose to create their own conventional, conditional, or knockin targeting vector(s), they can contract Transgenic Organism to perform the services of electroporation, tissue culture, microinjection, breeding of chimeras, and germline confirmation.
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